The 23-man squad was revealed in a statement published on the Nigeria Football Federation’s website on Thursday.
The duo of Victor Osimhen and Kelechi Iheanacho made the list.
Nigeria and Guinea Bissau clash at the Moshood Abiola National Stadium, Abuja from 5pm on Friday, March 24, and then make it a second date at the Estadio 24 de Setembro in Bissau on Monday, March 27, also starting from 5pm.
Spain-based defender Kenneth Omeruo returns, as midfield enforcers Wilfred Ndidi and Frank Onyeka, and Portugal-based defender Bruno Onyemaechi gets an opportunity to vie for shirts against Semi Ajayi, Bright Osayi-Samuel, Kevin Akpoguma and Calvin Bassey.
Portugal-based left back Zaidu Sanusi is also back, and usual suspects Alex Iwobi, Joe Aribo, Samuel Chukwueze, Moses Simon, Terem Moffi and Paul Onuachu are also called.
Daniel Bameyi is called alongside team goalkeeper Chijioke Aniagboso. In-form goalkeeper Victor Sochima, one of the reasons Rivers United FC have inched close to a CAF Confederation Cup quarter-final ticket, will contend for the number one shirt with Francis Uzoho.
The players are expected to start arriving at the team camp in Abuja on Sunday, March 19. Table-toppers Nigeria are on maximum six points from their defeats of Sierra Leone and Sao Tome and Principe, with the Djurtus in second place on four points from their defeat of Sao Tome and Principe and draw with the Leone Stars.
All the invited players:
Goalkeepers: Francis Uzoho (AC Omonia, Cyprus); Victor Sochima (Rivers United); Kingsley Aniagboso (Giant Brillars)
Defenders: Oluwasemilogo Ajayi (West Bromwich Albion, England); Bright Osayi-Samuel (Fenerbahce SK, Turkey); Kevin Akpoguma (TSG Hoffenheim, Germany); Kenneth Omeruo (CD Leganes, Spain); Calvin Bassey (FC Ajax, The Netherlands); Daniel Bameyi (YumYum FC); Zaidu Sanusi (FC Porto, Portugal); Bruno Onyemaechi (Boavista FC, Portugal).
Midfielders: Wilfred Ndidi (Leicester City, England); Frank Onyeka (Brentford FC, England); Alex Iwobi (Everton FC, England); Joe Aribo (Southampton, England).
Forwards: Kelechi Iheanacho (Leicester City, England); Ahmed Musa (Sivasspor K, Turkey); Samuel Chukwueze (Villarreal CF, Spain); Moses Simon (FC Nantes, France); Ademola Lookman (Atalanta BC, Italy); Terem Moffi (OGC Nice, France); Victor Osimhen (Napoli FC, Italy); Paul Onuachu (Southampton FC, England).