The Saudi Arabian side, looking to rebound from Friday’s Saudi Pro League defeat to arch-rivals Al Hilal, had already qualified for the knockout stages as Group E winners.
With progress secured and one matchday to spare, Al Nassr manager Luis Castro chose to leave Ronaldo out of the squad for the match in Dushanbe, while naming among his substitutes the likes of Sadio Mane, Aymeric Laporte, Otavio, and Anderson Talisca.
Istiklol took the lead at the Pamir Stadium just after the half-hour when midfielder Alisher Dzhalilov fired a fine low shot into the bottom corner.
The hosts, yet to win in this season’s competition, were hoping for three points to lift them off the bottom of the group.
However, Abdulrahman Ghareeb levelled five minutes into the second half, the Nassr winger converting a close-range volley at the second time of asking following an initial stop from Istiklol goalkeeper Rustam Yatimov.
Castro did send for Talisca, Nassr’s second-top scorer this season behind Ronaldo, in the 74th minute, and Ivory Coast midfielder Seko Fofana and Portugal winger Otavio late on, but the visitors could not snatch the win.
Nassr ended the group stage unbeaten, with four victories and two draws.
In the group’s other match, Qatar’s Al Duhail put an end to Persepolis’ chances of qualifying by recording a 2-1 victory at the Azadi Stadium in Tehran. This encounter stretched to a remarkable 107 minutes overall.
Michael Olunga scored the winner with seven minutes of normal time remaining, moments after Giorgi Gvelesiani missed a penalty for the home side.
The result did not impact the overall standings in the group, though, with two-time runners-up Persepolis finishing second and Duhail third.
In Group A, leaders Al Ain cemented their place at the summit with a 2-1 win away to Turkmenistan’s Ahal.
The victory in Ashgabat means the UAE side, winners of the competition in 2003, won five of their six matches.
Al Ain were guaranteed to go through to the last 16 as group winners.
Meanwhile, Saudi Arabia’s Al Fayha rounded off their campaign with a 4-1 victory over Uzbek club Pakhtakor to finish second in Group A.
The 10 group winners and the six best second-placed teams advance to the knockout rounds, with the East Asia side of the groups concluding next week.
The last 16 begins in February, while the competition concludes with a two-legged final in May.
AFP